Defining a novel role of GW182 in maintaining miRNA stability

As you will see, while the referees acknowledge that the finding that GW182 prevents miRNA degradation is potentially interesting, both referees 1 and 2 also point out that, as it stands, the manuscript is rather preliminary and requires significant strengthening before it can be considered for publication by EMBO reports. Both referees remark that endogenous miRNA levels need to be examined in response to GW182 knockdown, and referee 2 adds that alternative methods should be employed to investigate miRNA levels. Referee 1 further indicates that knockdown efficiencies should be demonstrated for XRN1/2 and TNRC6C, and referee 2 mentions that possible indirect effects of shRNA overexpression on miRNA stability should be excluded, or at least addressed.